mali transformation mediated by Agrobacterium tumefaciens. In this paper, we reported the construction of a binary vector pKO1-HPH containing a positive selective gene hygromycin phosphotransferase (hph), a reporter gene gfp conferring green fluorescent protein, and an efficient protocol for V. The pathogenic mechanism of the pathogen has not been studied extensively, thus a suitable gene marker for pathogenic invasion analysis and a random insertion of T-DNA for mutants are desirable. mali, the causal agent of valsa canker of apple, causes great loss of apple production in apple producing regions. mali.Ĭhen, Liang Sun, Gengwu Wu, Shujing Liu, Huixiang Wang, Hongkai Efficient transformation and expression of gfp gene in Valsa mali var.
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March 2023
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